- Program Director in Computational Biology, Center for Computational Science
- Walter G Ross Chair in Developmental Neuroscience
We use High Content Analysis to test thousands of genes and compounds on various primary neurons in culture to identify those that can promote or inhibit axon growth and regeneration. We use information theory and artificial intelligence approaches to identify targets.
We use RNASeq to uncover mRNAs and miRNAs relevant to axon regeneration. We are especially interested in transcription factors as tools to promote regeneration and use bioinformatic tools to uncover them.
To test hits from our in vitro screens we use models of spinal cord injury and optic nerve crush.
We work on ontology development and BD2K projects with Stephan Schürer, PhD in the Center for Computational Sciences and Ubbo Visser, PhD in Computer Sciences on the Bioassay Ontology project, the BD2K LIFE project, and the RegenBase project.
- Blackmore MG, Wang Z, Lerch JK, Motti D, Zhang YP, Shields CB, Lee JK, Goldberg JL, Lemmon VP, Bixby JL. Kru¨ppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract. Proc Natl Acad Sci U S A. 2012 109:7517-22. PMID: 22529377
- Hellal F, Hurtado A, Ruschel J, Flynn KC, Laskowski CJ, Umlauf M, Kapitein LC, Strikis D, Lemmon V, Bixby J, Hoogenraad CC, Bradke F. Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury. Science. 2011 331:928-31. PMID: 21273450
- Buchser WJ, Slepak TI, Gutierrez-Arenas O, Bixby JL, Lemmon VP. Kinase/phosphatase overexpression reveals pathways regulating hippocampal neuron morphology. Mol Syst Biol. 2010 6:391. PMID: 20664637
- Moore DL, Blackmore MG, Hu Y, Kaestner KH, Bixby JL, Lemmon VP, Goldberg JL. KLF family members regulate intrinsic axon regeneration ability. Science. 2009 326:298-301. PMID: 19815778
- Lemmon V, Farr KL, Lagenaur C. L1-mediated axon outgrowth occurs via a homophilic binding mechanism. Neuron. 1989 2:1597-603. PMID: 2627381
- Lagenaur C, Lemmon V. An L1-like molecule, the 8D9 antigen, is a potent substrate for neurite extension. Proc Natl Acad Sci U S A. 1987 84:7753-7. PMID: 3478724
For many years Dr. Lemmon studied development of the nervous system, especially the role of cell adhesion molecules and extracellular matrix molecules. His early work on L1cam showed that it promotes axon growth (PNAS, 1987, Neuron, 1989) and his cloning of human L1cam allowed geneticists to discover its role in causing X-linked hydrocephalus. His lab did extensive work on L1cam signaling and recycling in the context of axon growth. Work in his lab by Diane Snow and in collaboration with Jerry Silver revealed that CSPGs inhibit neurite growth (Exp Neurol. 1990). His lab was first to report that N-cadherin is a potent stimulator of oligodendrocyte maturation. Since coming to Miami he has studied axon regeneration.